Improved synthesis of firefly D-luciferyl-D-adenylate - A key intermediate of firefly bioluminescence.
نویسندگان
چکیده
منابع مشابه
OPTIMUM DESIGN OF STRUCTURES USING AN IMPROVED FIREFLY ALGORITHM
Nature-inspired search algorithms have proved to be successful in solving real-world optimization problems. Firefly algorithm is a novel meta-heuristic algorithm which simulates the natural behavior of fireflies. In the present study, optimum design of truss structures with both sizing and geometry design variables is carried out using the firefly algorithm. Additionally, to improve the efficie...
متن کاملβ‐Deuterium Isotope Effects on Firefly Luciferase Bioluminescence
A 5,5-d2 -luciferin was prepared to measure isotope effects on reactions of two intermediates in firefly bioluminescence: emission by oxyluciferin and elimination of a putative luciferyl adenylate hydroperoxide to dehydroluciferin. A negligible isotope effect on bioluminescence provides further support for the belief that the emitting species is the keto-phenolate of oxyluciferin and rules out ...
متن کاملEndotoxin assay by bioluminescence using mutant firefly luciferase.
The Limulus reaction is an application of the defense mechanism of horseshoe crab for endotoxin detection. Endotoxin is a component of the cell wall in the outer membrane of gram-negative bacteria, and causes fever or shock when it enters the human blood stream. For endotoxin detection, gel formation or turbidity of the coagulation factor chromogen or fluorescence-modified peptide is used. Howe...
متن کاملSite-directed mutagenesis of firefly luciferase: implication of conserved residue(s) in bioluminescence emission spectra among firefly luciferases.
The bioluminescence colours of firefly luciferases are determined by assay conditions and luciferase structure. Owing to red light having lower energy than green light and being less absorbed by biological tissues, red-emitting luciferases have been considered as useful reporters in imaging technology. A set of red-emitting mutants of Lampyris turkestanicus (Iranian firefly) luciferase has been...
متن کاملAn Improved Firefly Algorithm with Adaptive Strategies
Firefly Algorithm (FA) is a powerful swarm intelligence algorithm i spired by the flash phenomenon of the fireflies. However, it has weaknesses on optimizing high-dimensional problems. This paper presents an improved FA named Adaptive Firefly Algorithm (AFA). In the new algorithm, three strategies are proposed to improve its adaptability and overcome its weaknesses. The algorithm is tested on a...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1988
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.52.2803